In this class we will use PBS-Tween.  PBS (Phosphate Buffered Saline) is a very common reagent we use, and Tween-20 is a detergent and is also commonly used.

The recipe for PBS-Tween is:

• 0.13M NaCl (Mw: 58.44)
• 7mM Na₂HPO₄ (Mw: 141.96)
• 3mM NaH₂PO₄ (Mw: 137.99)
• Tween-20 (0.05% v/v)

You need to make 1.5 L PBS-Tween, in grams how much Na₂HPO₄ do you need (report to 2 decimal places)? g

How much Tween-20 (in mls) do you need to add (report to 2 decimal places)? ml