You have a cell suspension for which you need to determine the cell concentration.  You have stained your cells with trypan blue, using 20μl of cell suspension and 80μl of trypan blue.  Trypan blue stains dead cells blue, while live cells exclude this dye.

Your counts for each of the outer squares are:

1. Viable: 53; Dead: 12
2. Viable: 73; Dead:12
3. Viable: 74; Dead: 8
4. Viable: 70; Dead: 11

What is the concentration of viable cells in your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

You have a cell suspension for which you need to determine the cell concentration.  You have stained your cells with trypan blue, using 20μl of cell suspension and 20μl of trypan blue.  Trypan blue stains dead cells blue, while live cells exclude this dye.

Your counts for each of the outer squares are:

1. Viable: 53; Dead: 12
2. Viable: 73; Dead:12
3. Viable: 74; Dead: 8
4. Viable: 70; Dead: 11

What is the concentration of viable cells in your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

You have a cell suspension for which you need to determine the cell concentration.  You have stained your cells with trypan blue, using 20μl of cell suspension and 20μl of trypan blue.  Trypan blue stains dead cells blue, while live cells exclude this dye.

Your counts for each of the outer squares are:

1. Viable: 112; Dead: 26
2. Viable: 100; Dead:22
3. Viable: 109; Dead: 28
4. Viable: 107; Dead: 21

What is the concentration of viable cells in your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

You have a cell suspension for which you need to determine the cell concentration.

Your counts for each of the outer squares are:

1. 62
2. 68
3. 59
4. 68

What is the cell concentration for your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

What is 290.320001 to 3 significant figures?

What is 43.569 to 3 significant figures?

What is 2,855,634 to 3 significant figures?

You have a cell suspension for which you need to determine the cell concentration.

Your counts for each of the outer squares are:

1. 178
2. 181
3. 165
4. 175

What is the cell concentration for your sample? (You need to answer in standard notation, e.g. 4500 rather than 4.5×10³.)  (You must include units in your answer.)

What is 2.50086 to 3 significant figures?

In this class we will use PBS-Tween.  PBS (Phosphate Buffered Saline) is a very common reagent we use, and Tween-20 is a detergent and is also commonly used.

The recipe for PBS-Tween is:

• 0.13M NaCl (Mw: 58.44)
• 7mM Na₂HPO₄ (Mw: 141.96)
• 3mM NaH₂PO₄ (Mw: 137.99)
• Tween-20 (0.05% v/v)

You need to make 1.5 L PBS-Tween, in grams how much NaH₂PO₄ do you need (report to 2 decimal places)? g

How much Tween-20 (in mls) do you need to add (report to 2 decimal places)? ml