Refer to the bacterial growth data above presented in the workshop.

Using only the following terms, fill in the blanks (you can copy and paste the terms between commas):

acetate, acetate utilisation,  arginine, arginine utilisation, glucose, glucose utilisation,  glycerol, glycerol utilisation, histidine, histidine utilisation, destruction, no growth, robust growth, partial growth, DNA repair, carbon utilisation, nitrogen utilisation, gain no new information, determine a redundancy in function, observe that together they are essential genes

NOTE: not all the above terms are required, some may be used more than once and all terms must be spelled correctly (excluding the commas) to be marked as correct.

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When gene C is deleted we observe no growth when is the sole source in the media, with partial growth when exposed to only as a source in the media. When the gene C mutant is exposed to only glycerol as a carbon source we observe of the cells. The function of gene E appears to be related to . When observing the phenotype of the gene D mutant, it appears to be essential for .

From the result of the A&B double mutant across the growth conditions compared to the single mutants, we can .

From the result of the B&C double mutant across the growth conditions compared to the single mutants, we can .

Which of the following makeup of cellular membranes would be most likely best suited to HOT temperatures?

When experimenting on a transport system, you determine that the flow of solute is against the concentration gradient, and requires the direct application of ATP. The method of transport across the membrane is therefore likely:

Consider the above data when filling in the blanks for the following questions.

A scientist measures the cell volume of S. aureus cells in various NaCl solutions. She measures three different cell types, shown by the black (1st), blue (2nd) and red bars (3rd). One cell type is “wild type” S. aureus. The other two cell types have each had a gene deleted. These genes each encode a different sodium transporter membrane protein.

Which data most likely represents the cell volume of the wild type S. aureus?

In those cells that have expanded in comparison to the wildtype, the water has transferred from

Based on these data, the cell type represented by the red (3rd) bars

If a scientist carried out a growth experiment on a prokaryote in media without a nitrogen source you would expect:

 Dichotomous Key 2 

 Lab 2 Results 

• Best way: Transcribe the whiteboard data into an Excel file or the tables in your results sheet Word document. This makes it easier to use for your subsequent assignments.
• Quick way: Take a photo of the whiteboard containing all results.

Refer to the image below.

Brenton is looking at a sample of cells under the compound light microscope, using the x40 objective. To estimate the cell size, the correct formula he needs to use is: