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BIO1011 - Blueprints for Life - Clayton S1 2025

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 Scatterplot Graph 

Using the average absorbance values from the 8 replicates for each treatment, construct an XY scatterplot graph (using Excel) with trendlines using linear regression (R2) for each treatment. Upload this graph here.

NOTE: Students need to be proficient at constructing graphs (for future assessments) and this provides an opportunity to practice using the software while you can get assistance from your peers. TA’s will NOT assist with graph construction during the lab session.

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As with all spectrophotometric measurements, it is essential to use a blank.

From the list of reagents provided below, select all the components required to make up the blank.

Note: Multiple options may be correct. Marks are deducted for each incorrect selection.

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 Lab 3 Results 

Save your lab results! Upload your Lab 3 results here for future reference.

Choose one of the following:

  • Best way: Transcribe the whiteboard data into an Excel file or the tables in your results sheet document. This makes it easier to use for your subsequent assignments.
  • Quick way: Take a photo of the whiteboard containing all results.

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In reference to the experimental protocols, which of the following will be included in all experimental treatments (not including the blank)?

Note: Multiple options may be correct. Marks are deducted for each incorrect selection.

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In this experiment you will be investigating the photosynthetic electron transport chain by analysing the reduction of a dye in the presence of six (6) different treatments.

Based on the information presented in the prelab resources and activities, answer the following questions.

a) What is the dependent variable?

b) In the DCMU treatment, what is the independent variable?

c) This experiment analyses electron transport through the photosynthetic electron transport chain. Which of the following represents the positive control?

d) Which of the following represents the negative control?

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If photosynthesis is occurring in chloroplasts under white (natural) light, what would you expect to observe when you set up your sample and expose it to white light (i.e. what change will be observed)?

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 Prelab Activity 

Upload your prelab activity here. Remember that your hypothesis should make reference to all treatments and include a justification for each.

Once you have collated all the results from your lab session, indicate in your prelab activity table whether the results supported each component of your hypothesis or not.

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What aspect of photosynthesis are we specifically measuring with these experiments?

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In the following paragraph, please fill in the blanks with one of the terms in the following list, which are separated by commas.  Please note, the terms must be spelled exactly as shown, no punctuation marks or plural words are permitted, uppercase letters must not be used, you cannot use any word that is not listed.  The same word may be used more than once or not at all.

 

Aerobic respiration, anaerobic respiration, blood air threshold, blood oxygen threshold, decreasing, dive gas threshold, dive lactate threshold, heart rate, high, increasing, less than, low, metabolic rate, more than, oxidising, oxidative phosphorylation, oxygen, remaining constant, respiratory rate, water

 

The following graph shows the amount of gaseous oxygen in arterial blood, venous blood and alveoli of Emperor penguins undergoing a breath-hold dive.

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During region 1, the penguin is/are existing oxygen supplies within their circulatory system, while you would expect the blood lactate levels are by an order of 0.5 mmol/litre/min.  This continues for about 5-6 minutes when the is reached, when the penguin takes on more when entering region 2.  During region 2, you can see blood lactate levels is/are by 0.5 mmol/litre/min for the remainder of the dive.  During region 2, you would expect blood oxygen levels to remain quite .  This same phenomenon was observed during the workshop, when the presenter’s dropped while holding his head underwater.

 

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Please answer the following questions by choosing the correct response from the drop down menus:

 

Q1.  Following a typical aerobic exercise training program, we would not expect a professional athlete to display

Q2.  During a dive, we would expect heart rate to

and lactate production to
.  This is because
.

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