Q6.The first 10 nucleotides of the first exon of the ProfX gene are 5’ ATG GGT TCC T 3’. What is the sequence of the first 10 nucleotides of transcribed mRNA in the pre-mRNA transcript?

Q4. What type of splicing most likely produced mRNA transcript B?

Q5.

RT-PCR

was performed on the enriched plant RNA using region-specific

primers for the

Q3. What type of splicing most likely produced mRNA transcript A?

Q2.

The researcher treated the

second batch of RNA with a MIXrobe kit that removes poly-adenylated RNA

transcripts using magnetic beads. Explain

why the MIXrobe kit does not remove bacterial mRNA. (1-2 sentences) (2 marks)

Q1. 

The researcher treated one batch

of the extracted RNA with the reagent Captcha which specifically binds to

7-methylguanosine. 

What personal protective equipment are you required to bring / wear to this practical? Select all that apply.

Which of the following reagents can cause eye irritation and/or eye damage? Select all that apply.

Which of the following reagents can cause skin irritation, damage or burns? Select all that apply.

A 260/280 ratio of ____ is generally accepted as pure for DNA.