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MCB2011 - Molecular biology and the cell - S1 2025

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Which of the following hazards are associated with neutral red dye? Select all that apply.

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The vital dye neutral red is internalised within:

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The _______________ marks the site of fertilisation and embryo development in a chick egg.

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Which of the following labels on the E3.5 embryo is incorrect?

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Which of the following labels on the E2.5 embryo is incorrect?

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In this practical apoptotic cells will be visualised using which dye?

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How long does it take for a chick to go from fertilisation to hatching?

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During the phylotypic stage, vertebrate embryos from different species ______________________________.

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The researcher

captured the electron micrograph image below which shows the release of albumin

from a secretory vesicle within a hepatocyte into the extracellular

environment.

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Identify the donor and target membranes for the secretory vesicle. (2

marks)

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The import of acyl-CoA dehydrogenase into the mitochondrion is defective

in cell line HEP-M1. The researcher used subcellular fractionation and western

blotting to detect acyl-CoA dehydrogenase in different subcellular

compartments. The western blotting results for normal healthy hepatocytes and the

HEP-M1 cell line are summarised in the figure below.

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Which of the

following mutations (A, B, C) is most likely present in the HEP-M1 cell line?

Use the western blotting results and your knowledge of the mitochondrial

protein import process to justify your response. (2-3 sentences) (2 marks)

A. The acyl-CoA

dehydrogenase mitochondrial signal sequence is mutated

B. The

receptor protein in the TOM complex is mutated

C. The

translocation channel in the TIM23 complex is mutated

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