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Which of the following hazards are associated with neutral red dye? Select all that apply.
The vital dye neutral red is internalised within:
The _______________ marks the site of fertilisation and embryo development in a chick egg.
Which of the following labels on the E3.5 embryo is incorrect?
Which of the following labels on the E2.5 embryo is incorrect?
In this practical apoptotic cells will be visualised using which dye?
How long does it take for a chick to go from fertilisation to hatching?
During the phylotypic stage, vertebrate embryos from different species ______________________________.
The researcher
captured the electron micrograph image below which shows the release of albumin
from a secretory vesicle within a hepatocyte into the extracellular
environment.
Identify the donor and target membranes for the secretory vesicle. (2
marks)
The import of acyl-CoA dehydrogenase into the mitochondrion is defective in cell line HEP-M1. The researcher used subcellular fractionation and western blotting to detect acyl-CoA dehydrogenase in different subcellular compartments. The western blotting results for normal healthy hepatocytes and the HEP-M1 cell line are summarised in the figure below.
Which of the following mutations (A, B, C) is most likely present in the HEP-M1 cell line? Use the western blotting results and your knowledge of the mitochondrial protein import process to justify your response. (2-3 sentences) (2 marks)
A. The acyl-CoA dehydrogenase mitochondrial signal sequence is mutated B. The receptor protein in the TOM complex is mutated C. The translocation channel in the TIM23 complex is mutated
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