Use the picture below to answer the following questions. You want to ...
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Use the picture below to answer the following questions.
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You want to study three genes, so you create three fusion proteins. The first gene (gene1) is fused to green fluorescent protein (GFP), the second gene (gene2) is fused to red fluorescent protein (RFP) and third protein (gene3) is fused to a cyan fluorescent protein (CFP). You study a cross section view of a single cell under the microscope.What type of protein is encoded by gene1?You modify gene1 so that its protein product is not targeted to the rough endoplasmic reticulum (ER), but is still translated by ribosomes. What will be the final destination of the protein produced by this modified gene?
You express your gene2/RFP fusion gene inside several types of human cell. You find that this protein is most abundant in a muscle cell - the whole cell appears to be red. What organelle is the product of gene2 targeted to?
You are puzzled when you look at the gene3 fusion product - when you study the cell over a period of time, you see that the cyan fusion protein is produced, transported to an organelle, but then that the colour slowly disappears over time. What organelle do you think the gene3/CFP fusion protein is being targeted to?
Please answer all parts of the question.
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