During a pulse-chase experiment with secreted proteins, the proteins are synthesized for a short “pulse” time with radioactive or fluorescent amino acids to label the proteins. During the “chase” period, unlabeled amino acids are added, so any additional proteins synthesized are not labeled. The labeled proteins can then be monitored over time. You complete a pulse-chase experiment to monitor the secretion of a protein from the cell. Which of the following correctly lists the order of locations of the protein during the chase period?

Question

Accepted Answer

endoplasmic reticulum → transport vesicle → Golgi → transport vesicle → secreted

Answer

Golgi → transport vesicle → endoplasmic reticulum → transport vesicle → secreted

Answer

endoplasmic reticulum → transport vesicle → Golgi → cytosol → secreted

Answer

nucleus → endoplasmic reticulum → cytosol → Golgi → transport vesicle → secreted

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During a pulse-chase experiment with secreted proteins, the proteins are synthes...

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