a sterile serological pipette to inoculate culture fluid onto the

surface of an agar plate and then a sterile bent glass rod to spread the

inoculant over the agar surface.

a sterile cotton swab to inoculate culture fluid onto the surface of an agar plate.

a sterile serological pipette to pipette culture fluid into a sterile Petri dish which is then mixed with molten agar.

a sterile cotton swab to inoculate culture fluid into a sterile Petri dish.

a sterile serological pipette to pipette culture fluid into a sterile Petri dish and then a sterile bent glass rod to spread the

inoculant the surface.

0.45mm

0.54µm

0.75mm

0.45µm

Take 1ml from this tube and inoculate 9mls of liquid in a second tube.

Take 1ml from the second tube and inoculate 9mls of liquid in a third tube.

Take 1ml from this tube and inoculate 9mls of liquid in a second tube.

Take 1ml from the second tube and inoculate 9mls of liquid in a third tube.

Take 1ml from the third tube and inoculate 9mls of liquid in a fourth tube.

Take 1ml from the fourth tube and inoculate 9mls of liquid in a fifth tube.

Take 1ml from this tube and inoculate 9mls of liquid in a second tube.

Take 1ml from the second tube and inoculate 9mls of liquid in a third tube.

Take 1ml from the third tube and inoculate 9mls of liquid in a fourth tube.

Take 1ml from the fourth tube and inoculate 9mls of liquid in a fifth tube.

Take 1ml from the fifth tube and inoculate 9mls of liquid in a sixth tube.

Take 1ml from this tube and inoculate 9mls of liquid in a second tube.

Take 1ml from the second tube and inoculate 9mls of liquid in a third tube.

Take 1ml from the third tube and inoculate 9mls of liquid in a fourth tube.

can only be done using dry heat.

destroys most life forms.

destroys all life forms.

can only be done using moist heat.

tip

digital volumeter

push button

tip ejector

thumb wheel

10^-5

10^-1

10^-2

10^-4

1:100000

1:10000

1:100

1:1000

1:10

add 1ml of sample into 99mls of sterile broth.

remove 1ml of broth from 100mls of sterile sample.

add 1ml of sample into 100mls of sterile broth.

remove 1ml of broth from 99mls of sterile sample.