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A researcher extracts protein from cultured hepatocytes (i.e. liver cells) and t...

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A researcher extracts protein from cultured hepatocytes (i.e. liver

cells) and treats the protein sample with sodium dodecyl sulphate (SDS) and

β-mercaptoethanol (BME). The protein sample is loaded onto a polyacrylamide gel

and a western blot is performed as per the steps described in the relevant online

learning module (OLM 6.2). The researcher uses a primary antibody that specifically

binds to the N-terminal region of acyl-CoA dehydrogenase.

Image failed to load

Examine the

western blotting results shown in the image above (A, B, C). Which result would

you expect to obtain for this experiment?

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