In this question you need to prepare a sodium phosphate standard curve across f...
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In this question you need to prepare a sodium phosphate standard curve across five tubes, with an amount of phosphate ranging from 0 μmol (in Tube 1) to 1.0 μmol (in Tube 5). The amount should increase evenly in 0.25 μmol increments.
You are provided with a solution of 1.25 mM sodium phosphate. Each tube must also contain 400 μL of reaction mix. The total volume in each tube should be 1.5 mL.
With this information, you should be able to complete the table below.
| TUBE 1 | TUBE 2 | TUBE 3 | TUBE 4 | TUBE 5 | |
|---|---|---|---|---|---|
| Sodium phosphate | μmol | μmol | μmol | μmol | μmol |
| 1.25 mM sodium phosphate | mL | mL | mL | mL | mL |
| Reaction mix | mL | mL | mL | mL | mL |
| Water | mL | mL | mL | mL | mL |
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