When added to a solution at an enzyme concentration of 0.1 μM , chymotrypsin catalyses the hydrolysis of a peptide substrate with the following V max and K m values.

V max = 300 μM s -1
K m = 3 mM

If the same reaction is repeated but using a chymotrypsin concentration of 0.2 μM , what will be the values of V max , K m , and k cat under these new conditions ?

Question

When added to a solution at an enzyme concentration of  0.1 μM , chymotrypsin catalyses the hydrolysis of a peptide substrate with the following V max  and K m  values. 
 
 V max  = 300 μM s -1 
 K m  = 3 mM 
 
 If the same reaction is repeated but using a chymotrypsin concentration of  0.2 μM , what will be the  values of V max , K m , and k cat  under these new conditions ?

Answer

V max  = 300 μM s -1 ; K m  = 6 mM; and k cat  = 6000 s -1

Answer

V max  = 300 μM s -1 ; K m  = 6 mM; and k cat  = 3000 s -1

Answer

V max  = 600 μM s -1 ; K m  = 3 mM; and k cat  = 3000 s -1

Answer

V max  = 600 μM s -1 ; K m  = 3 mM; and k cat  = 6000 s -1

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When added to a solution at an enzyme concentration of 0.1 μM , chymotrypsin ca...

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