You are given 10ml of a 10 -1 dilution and are required to make a 10 -5 dilution. How do you do this? In addition to the 10ml sample, you have unlimited supplies.

Question

You are given 10ml of a 10 -1  dilution and are required to make a 10 -5  dilution. How do you do this? In addition to the 10ml sample, you have unlimited supplies.

Accepted Answer

Take 1ml from this tube and inoculate 9mls of liquid in a second tube. 
 Take 1ml from the second tube and inoculate 9mls of liquid in a third tube. 
 Take 1ml from the third tube and inoculate 9mls of liquid in a fourth tube. 
 Take 1ml from the fourth tube and inoculate 9mls of liquid in a fifth tube.

Answer

Take 1ml from this tube and inoculate 9mls of liquid in a second tube. 
 Take 1ml from the second tube and inoculate 9mls of liquid in a third tube.

Answer

Take 1ml from this tube and inoculate 9mls of liquid in a second tube. 
 Take 1ml from the second tube and inoculate 9mls of liquid in a third tube. 
 Take 1ml from the third tube and inoculate 9mls of liquid in a fourth tube. 
 Take 1ml from the fourth tube and inoculate 9mls of liquid in a fifth tube. 
 Take 1ml from the fifth tube and inoculate 9mls of liquid in a sixth tube.

Answer

Take 1ml from this tube and inoculate 9mls of liquid in a second tube. 
 Take 1ml from the second tube and inoculate 9mls of liquid in a third tube. 
 Take 1ml from the third tube and inoculate 9mls of liquid in a fourth tube.

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You are given 10ml of a 10 -1 dilution and are required to make a 10 -5 diluti...

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