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BCH2011 - Structure and function of cellular biomolecules - S1 2025

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You took 3 mL of a stock solution of sodium phosphate (25 mM) and mixed it with 12 mL of water to make solution A.

You then took 1 mL of a solution A and mixed it with 1 mL of water to make solution B. 

You then took 1 mL of a solution B and mixed it with 4 mL of water to make solution C.

Calculate the concentration (in mM) of sodium phosphate in each of these solutions.

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5 mL of a stock solution of 250 mM Tris buffer is diluted to a final volume of 25 mL.

What is the final concentration of the diluted Tris buffer (in units of mM)?

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How many micromoles (μmol) of ATP are present in 6 mL of 5 mM ATP solution?

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A sample contains 700 μg of NaOH.

How many milligrams (mg) is this?

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A patient has been admitted to hospital and a blood test has shown his glucose level is 45 mg per 55 mL.

Calculate the molarity (in mM, to two decimal places) of the glucose in the patient's blood.

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Shown below is a DNA duplex sequence, including both the coding (top) and non-coding (bottom) strands. 

Which one of the following pairs of oligonucleotide primers would be most suitable for amplifying (by PCR) the region of this duplex shaded in yellow?

[Note: You are not expected to estimate Tm values for these primers. If they have the correct sequence to anneal in the correct position, you should assume that they will.]

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Shown below are three short DNA duplexes.

What is the correct order of stability of these duplexes (from most stable to least stable)

[Note: You do not need to calculate Tm values. You should be able to determine the order of stability “by eye”.]

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The image below is a helical wheel representation of part of an alpha helix (top-down view), with each amino acid a numbered circle.

Without knowledge of any R groups, non-covalent interactions between which ONE of the following pairs of amino acids will likely help stabilise the helix?

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How many peptide bonds are there in the following peptide sequence?

MetCysGlySerProLeu

(Enter a number in digits with no additional characters)

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Why is glycine (shown below) the only non-chiral amino acid?

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