The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the K_m value (in units of mM) for this data set.

Note: You should type the number only, with at least one decimal place (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the V_max value (in units of μM/s) for this data set.

Note: You should type the number only (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the V_max value (in units of μM/s) for this data set.

Note: You should type the number only (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the V_max value (in units of μM/s) for this data set.

Note: You should type the number only (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the K_m value (in units of mM) for this data set.

Note: You should type the number only (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the K_m value (in units of mM) for this data set.

Note: You should type the number only, with at least one decimal place (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the K_m value (in units of mM) for this data set.

Note: You should type the number only (not the units or any other characters).

The graph below displays a set of kinetics data for an enzyme that follows Michaelis-Menten kinetics.

Estimate the V_max value (in units of μM/s) for this data set.

Note: You should type the number only (not the units or any other characters).

Shown below is a binding curve for a drug binding to a single binding site on a target protein.

From this curve estimate the fraction of protein molecules that will be bound to the drug when the concentration of the drug added to the solution is 20 nM.

Your answer should be given as a number, e.g., 0.5, not 50% (do not type any additional characters)

A heterodimeric protein is composed of two subunits that have similar structures. Each subunit is able to bind to the same ligand molecule. Binding of the ligand to one subunit induces a conformational change in the protein such that the affinity of the ligand for the second subunit increases.

Which of the following binding curves is/are likely to represent this type of protein-ligand interaction?